Description
ASNS, also known as Asparagine synthetase, is a one of cytoplasmic enzyme that generates asparagine from aspartate. It mainly distributes in mammalian organs, but basal expression is low in tissues other than the exocrine pancreas. This protein plays a role of cell growth, and its mRNA content is linked to changes in the cell cycle. Its mRNA is upregulated in response to the asparagine depletion, some of the toxic effects of themedication on normal cell activity. Above average presence of asparagine synthetase in certain leukemia strains has been linked to be a significant contributing factor of chemotherapy resistance, particularly to thechemotherapy drug, L-asparaginase. Also, it functions as potential biomarker for ovarian cancer, potential role in solid tumor metastasis. Recombinant mouse ASNS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques